Journal: International Journal of Molecular Sciences

Article Title: Combination Therapies Targeting Apoptosis in Paediatric AML: Understanding the Molecular Mechanisms of AML Treatments Using Phosphoproteomics

doi: 10.3390/ijms24065717

Figure Lengend Snippet: Validation of double and triple combinations in the paediatric AML cell lines. ( A ) Flow cytometry analysis of the annexin V-positive cell population for Kasumi-1 and MV4-11 cells following 72 h treatment as single agents and as a combination. ( B ) Western blot analysis of PARP, cleaved-PARP, procaspase-9, cleaved caspase-9, procaspase-3, and cleaved caspase-3 in Kasumi-1 and MV4-11 cell lines following 72 h treatment as single agents and as a combination. GAPDH was used as a loading control. All results shown are representative of three independent experiments. ( C ) Reactive oxygen species quantification following 72 h treatment as single agents and as a combination. Stained cells were analysed for fluorescence, measured using a Synergy HTX Multi-Mode Micro-Plate reader. We used 5 mM H2O2 and 5 mM N-acetyl cysteine (NAC) anti-oxidant as positive and negative controls respectively. * = p < 0.05, ** = p < 0.005 values of significance of treatments compared to controls were calculated using the paired t -test.

Article Snippet: The supernatant was removed, and the cells were re-suspended in 300 µL of binding buffer and transferred to a flow cytometry tube (BD Biosciences).

Techniques: Flow Cytometry, Western Blot, Staining, Fluorescence

Journal: Journal of Virology

Article Title: A Species-Specific Amino Acid Difference in the Macaque CD4 Receptor Restricts Replication by Global Circulating HIV-1 Variants Representing Viruses from Recent Infection

doi: 10.1128/JVI.02176-12

Figure Lengend Snippet: Effect of single residue changes in CD4 on HIV-1 infection. (a) Infection of 293T cells transiently expressing huCCR5 and huCD4, ptCD4, or huCD4 and ptCD4 point mutants with GFP reporter pseudoviruses bearing the Q23-17 Env. The y axis represents viral infection relative to huCD4. Error bars represent the standard deviation of the mean from duplicate independent experiments. n.d., not determined due to insufficient CD4 expression. (b) Flow cytometric analysis of 293T cells transiently expressing huCCR5 and huCD4 or ptCD4 receptors. The y axis represents CD4 expression, and the x axis represents CCR5 expression as determined by flow cytometry. The CD4 receptor that is expressed in the cells is labeled at the top of each plot. These plots are representative of at least three independent experiments. (c) Infection of Cf2Th/syn CCR5 cells stably expressing ptCD4 I39N relative to huCD4-expressing cells with GFP pseudoviruses expressing the YU-2 Env and acute/early Envs from subtypes A to D (as denoted on the x axis). Error bars represent the standard deviation of the mean from two independent experiments.

Article Snippet: The fixed cells were transferred to flow cytometry tubes (BD Falcon) that were centrifuged at 800 × g for 5 min.

Techniques: Residue, Infection, Expressing, Standard Deviation, Flow Cytometry, Labeling, Stable Transfection

Journal: Research in Pharmaceutical Sciences

Article Title: Anti- Toxoplasma gondii activity of 5-oxo-hexahydroquinoline derivatives: synthesis, in vitro and in vivo evaluations, and molecular docking analysis

doi: 10.4103/1735-5362.293515

Figure Lengend Snippet: Flow cytometry analysis of Toxoplasma gondii tachyzoites exposed to different concentrations of Z1, Z2, Z3, Z4, negative and positive controls.

Article Snippet: The cell suspension was transferred into polystyrene flow cytometry tubes (BD Falcon, USA).

Techniques: Flow Cytometry